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A high-performance liquid chromatographic (HPLC) method for the determination of norfloxacin in blood has been developed. The method involves the use of a diethylaminoethyl semipreparative column and the measurement of the intensity of the fluorescence of the 4-methoxybenzoic acid (4-MBA) derivative of the drug. Blood is deproteinized, then applied to the HPLC column and collected for analysis. The drug and the derivative are eluted with aqueous buffer containing tetrabutylammonium hydroxide. The fluorescence of the 4-MBA derivative is determined at wavelengths of 290 and 335 nm. The sensitivity and precision of the method were established using pooled blood. The recovery of norfloxacin in pooled blood was 99%. This method has been applied successfully to the determination of norfloxacin in blood.# Copyright 2016 The Kubernetes Authors.
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